{"id":14559,"date":"2023-11-13T04:22:19","date_gmt":"2023-11-13T04:22:19","guid":{"rendered":"https:\/\/galenica.cl\/english\/?post_type=product&#038;p=14559"},"modified":"2024-01-10T17:39:22","modified_gmt":"2024-01-10T17:39:22","slug":"5-nucleotidase-5-nt","status":"publish","type":"product","link":"https:\/\/galenica.cl\/english\/product\/5-nucleotidase-5-nt\/","title":{"rendered":"5\u2019-Nucleotidase (5\u2019-NT)"},"content":{"rendered":"<div id=\"cmsmasters_row_\" class=\"cmsmasters_row cmsmasters_color_scheme_default cmsmasters_row_top_default cmsmasters_row_bot_default cmsmasters_row_boxed\">\n<div class=\"cmsmasters_row_outer_parent\">\n<div class=\"cmsmasters_row_outer\">\n<div class=\"cmsmasters_row_inner\">\n<div class=\"cmsmasters_row_margin\">\n<div id=\"cmsmasters_column_\" class=\"cmsmasters_column one_first\">\n<div class=\"cmsmasters_column_inner\"><div class=\"cmsmasters_text\">\n<p>\nDiazyme\u2019s 5\u2032-Nucleotidase Assay is linear from 0 \u2013 300 U\/L (R\u00b2 &gt;0.99) with intra-assay CV% of &lt; 2% and an inter-assay CV% of \u2264 4%. 5\u2032-Nucleotidase assay is not affected by interfering substances such as serum bilirubin up to 40 mg\/dL, hemoglobin up to 500 mg\/dL, triglycerides up to 1250 mg\/dL, ascorbic acid up to 20 mg\/dL, and alkaline phosphatase up to 1250 U\/L.<\/p>\n<p>Assay Principle<br \/>\nThe 5\u2032-NT assay is based on the enzymatic hydrolysis of 5\u2032-monophosphate (5\u2032-IMP) to form inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is further reacted with N-Ethyl- N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4- aminoantipyrine (4-AA) in the presence of peroxidase (POD) to generate quinone dye which is monitored in a kinetic manner.<\/p>\n<p>Intended use<br \/>\nThe 5\u2032-Nucleotidase (5\u2032-NT) assay kit is for the determination of 5\u2032-NT activity in human serum samples.<\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Diazyme\u2019s 5\u2032-Nucleotidase Assay is linear from 0 \u2013 300 U\/L (R\u00b2 &gt;0.99) with intra-assay CV% of &lt; 2% and an inter-assay CV% of \u2264 4%. 5\u2032-Nucleotidase assay is not affected by interfering substances such as serum bilirubin up to 40 mg\/dL, hemoglobin up to 500 mg\/dL, triglycerides up to 1250 mg\/dL, ascorbic acid up to 20 mg\/dL, and alkaline phosphatase up to 1250 U\/L.<\/p>\n<button data-id=\"14544\" data-post_id=\"14559\" data-form_style=\"1\" data-allow_outside_click=\"1\" data-width=\"500px\" class=\"wpb-pcf-form-fire wpb-pcf-btn-large wpb-pcf-btn wpb-pcf-btn-default\">More information<\/button>\n","protected":false},"featured_media":14574,"comment_status":"closed","ping_status":"closed","template":"","meta":{"content-type":""},"product_cat":[15],"product_tag":[165],"class_list":["post-14559","product","type-product","status-publish","has-post-thumbnail","hentry","product_cat-uncategorized","product_tag-diazyme"],"_links":{"self":[{"href":"https:\/\/galenica.cl\/english\/wp-json\/wp\/v2\/product\/14559","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/galenica.cl\/english\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/galenica.cl\/english\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/galenica.cl\/english\/wp-json\/wp\/v2\/comments?post=14559"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/galenica.cl\/english\/wp-json\/wp\/v2\/media\/14574"}],"wp:attachment":[{"href":"https:\/\/galenica.cl\/english\/wp-json\/wp\/v2\/media?parent=14559"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/galenica.cl\/english\/wp-json\/wp\/v2\/product_cat?post=14559"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/galenica.cl\/english\/wp-json\/wp\/v2\/product_tag?post=14559"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}